Coding

Part:BBa_K3629021:Design

Designed by: Arshia Mostoufi   Group: iGEM20_Calgary   (2020-10-12)


mCherry codon optimized for Yarrowia lipolytica


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Below is the codon-optimized sequence of Yarrowia lipolytica mCherry for use in any expression construct. However, we have also made our own expression construct BBa_K3629025, designed to allow for optimal expression of mCherry in Yarrowia lipolytica using the TEfin promoter BBa_K3629001 and the XPR2 terminator BBa_K3629004.

Source

Original sequence obtained from https://www.fpbase.org/protein/mcherry/

References

1. [online] https://www.fpbase.org/protein/mcherry/ (Accessed October 26, 2020)