Coding
Part:BBa_K3629021:Design
Designed by: Arshia Mostoufi Group: iGEM20_Calgary (2020-10-12)
mCherry codon optimized for Yarrowia lipolytica
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Below is the codon-optimized sequence of Yarrowia lipolytica mCherry for use in any expression construct. However, we have also made our own expression construct BBa_K3629025, designed to allow for optimal expression of mCherry in Yarrowia lipolytica using the TEfin promoter BBa_K3629001 and the XPR2 terminator BBa_K3629004.
Source
Original sequence obtained from https://www.fpbase.org/protein/mcherry/
References
1. [online] https://www.fpbase.org/protein/mcherry/ (Accessed October 26, 2020)